iNap

Pre-made plasmids expressing iNaps, highly responsive sensors for NADPH. Variants of iNaps have different affinity, enabling them to be used in cells or subceullar organells with various NADPH levels. 

1\iNap sensor. The original iNap sensors is excited by  420 and 485 nm and emit green fluorescence. The fluorescence ratio of SoNar when excited at 420 and 485 nm reports NADPH redox state.  SoNar’s fluorescence when excited at 485 nm is sensitive to pH, whereas its fluorescence excited at 420 nm is pH-resistant.

2\iNapc, the pH- control sensor for both SoNar and iNap sensor. iNapc control sensor has similar structure and fluorescence as SoNar sensor, however it does not respond to NADPH redox state.  iNapc sensor is the ideal control sensor to normalized the effects of pH fluctuation during the time course of measurements. 

3\iNap-mCherry fusion. Fusion of SoNar to a red fluorescent protein allows measurement on the basis of the green/red fl uorescence ratio (i.e., 420/590-nm ratio), which is pH-resistent, but at the expense of lower sensor's dynamic range. 

For precise measurement of NADPH level, it is very important and neccessary to use iNapc to correct the potential pH effects for live cell studies or in vitro studies.  We recommend customers to purchase iNapc together with your order of iNap sensors.  

Promotion: For iNap sensor plasmids, customers may enjoy our buy one and get the second plasmid 50% off promotion policy. Effective during check out.